Isolation and Identification Pathogenic Bacteri with Special Regard to Staphylococcus auerus Associate with Cutaneous leishmaniasis at Thi – Qar Province
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2017-11-18
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Abstract
The present study is designed to isolated and identification the
pathogenic bacteria associated with Cutaneous leishmaniasis. Cutaneous
leishmaniasis (CL) is a parasitic disease characterized by single or
numerous ulcerations. This was study performed in Iraqi in Shatra
general hospital and Al-hussein teaching hospital in Thi-Qar province
2016-2017.
Total of 370 samples of suspected patients were taken and after
examining them using geimsa stain , the positive were 186 males and
126 females then sterilize the area surrounding for ulcer , all samples
were taken to (swab transport media) ,culture to MacConkey agar and
blood agar after 24 hr of incubation at 37C ,after diagnosed by
biochemical testing then species determined by API system. The positive
results on culture media 92/312 , males 62 (67.4%) , female 30 (32.6 %).
The total bacteria isolated from the lesions were in males and female
staph auerus 52 cases (56.5%), Escherichia coli 8 cases(8.6%) ,
Bacillus cereus 6 cases(6.5%) , Pseudomonas. aeruginesa 5 cases (5.4%
), Strep. Pyogenes 6 cases(6.5%) ,Strepto.agalactiae 3 cases
(3.3%).While other that isolated in males only and did not appear in
females were aerobic bacteria ,Listeria moncytogenes 3 cases(3.4%) ,
Actinobacteria .spp 2 cases(2.2%) ,Klebsiella. Pneumonia 2 cases
(2.2%) , Rodococcus .equi 1 cases (1.1%) , and anaeroberia bacteria
,Peptococcus niger 2 cases (2.2%) ,Peptostreptococcus .spp 2 cases
(2.2%) .
Then find bacteria that more presence more with cutaneous
Leishmaniasis ,were it was staph. aureus therefore it were objected study
antibiotic resistant .The present study demonstrates that Staph. aureus
isolates were resistant to methicillin 50 (96.15%), penicillin 48 (92.30%)
, Clindamycin 37 (71.15%), ciprofloxacin 30 (57.69%) , Gentamicin 30
(57.69%),Amoxicalave 29 (55.76%), Azithromycin 25 (48.07%) ,
Amoxicillin 22 (42.30%), Tetracycline 21 (40.38%), Rifampin 10
(19.23%) and Vancomycin all staph .auerus are sensitive to Vancomycin
The all 52 Staph. aureus isolates were further examined using polymerase
chain reaction (PCR) for detection of (16SrRNA ,mecA and PVI gene ).
16SrRNA gene which were responsible for diagnosis ,where all isolates were
positive to was 52\52 (100%) , mecA gene which were responsible for
methicillin resistance this was detected in 52\52 (100%) . of Staph. aureus
isolates and a part of virulence factors PVL gene was detected in
46/52(88.46%) of Staph. aureus isolates which are responsible to kill the
leukocytes of human and other animals