Potential benefits of Ocimum basilicum extract on protein expression on ALCL3 induced rats models of Alzheimer's disease.
| dc.contributor.author | مروه عامر شعلان | |
| dc.contributor.editor | أ.د. ليلى محسن مهدي | |
| dc.date.accessioned | 2024-11-03T08:36:53Z | |
| dc.date.available | 2024-11-03T08:36:53Z | |
| dc.date.issued | 2024-05-16 | |
| dc.description.abstract | Summary Alzheimer’s Disease (AD) is a progressive neurodegenerative disorder considered by oxidative stress, neuroinflammation and synaptic dysfunction, which caused in part by abnormal accumulation of senile plaques and neurofibrillary tangles outside and inside the cerebral neurons, respectively. The Aluminum Chloride( AlCl3) exposure led to the formation of amyloid beta (Aβ) plaques in rats hippocampus; however, none of the tested drugs caused a significant reduction in amyloid burden at the selected doses. The objective of present study is to evaluate the neuroprotective potential effect of Ocimum basilicum extract on oxidative stress status, Amyloid beta (AB) , Tau protein expression in rat induced Alzheimer's disease. Fifty adult male Sprague Dawley albino rats were used in this study. The animals used were divided into five groups (10 rats each) as follows: Group I: Rats (negative group) were received normal diet and tap water to end of experiment. Group II: Rats (positive group) were induced 17 mg/kg orally and normal diet for one month. Group III: AD-induced rats and treated with 0.3 mg/kg Rivastigmine for one month. Group IV: AD-induced rats and treated by 250 mg/kg Ocimum basilicum for one month. Group V : AD-induced rats and treated by 500 mg/kg Ocimum basilicum for one month. At the end of experiment, Serum levels were used to determine Super oxide dismutase (SOD)and Malondyhide(MDA). Then, animals was scarified to obtain brain to determine Beta amyloid and Tau protein by immunohistochemistry assay. Results show that serum SOD is significantly increased and MDA was decreased when plant leaves extract administrated orally four weeks with decreased of aggregation of tau protein expression. The results showed that deposition of amyloid protein expression was increased in positive group (AD induced rats) . Histological changes were observed in brain tissues of AD rats. Minor deposition of amyloid protein in cerebral parenchyma was observed. However, the high dosing of the plant leaves extract (500 mg/kg) was more powerful than the low treatment with low dose (250 mg/kg) by decreasing accumulation of amyloid protein expression. Research results imply that by enhancing memory function, ocimum basilicum can reduce symptoms and stop the severity of AD increasing. It can be concluded that OB leaves were alleviated the memory impairment and learning abilities and can relieve symptoms and prevent the progression of the AD severity due to antioxidants activity of flavonoids, tannins and terpenoids. | |
| dc.identifier.uri | https://dspace.utq.edu.iq/handle/123456789/168 | |
| dc.language.iso | en | |
| dc.title | Potential benefits of Ocimum basilicum extract on protein expression on ALCL3 induced rats models of Alzheimer's disease. | |
| dc.title.alternative | على التعبير الروتيني على نماذج الفئران المستحثة لمرضى الزهايمر Ocimum basilicum الفوائد المحتملة لمستخلص | |
| dc.type | text::thesis::master thesis | |
| oairecerif.author.affiliation | كلية التربية للعلوم الصرفة - قسم علوم الحياة | |
| oairecerif.editor.affiliation | جامعة ذي قار - كلية التربية للعلوم الصرفة -قسم علوم الحياة |